DTX3L Knockout cell line (HUVEC)

DTX3L Knockout cell line (HUVEC) Order

Catalog#

YKO-KP179

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

DTX3L Knockout cell line (HUVEC)

Cell Morphology

Epithelial-like, adherent

Passage ratio

1：2-1：3

Species

Human

Gene Name

DTX3L

Gene ID

151636

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

Target region

Knockout region: Exon 2,Exon 3 (based on transcript DTX3L-201)
Validation: PCR+Sanger Sequencing

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the DTX3L gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HUVEC cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, DTX3L gene knockout DTX3L Knockout cell line (HUVEC) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

DTX3L Knockout cell line (HUVEC)

Catalog#

YKO-KP179

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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